How to Use Your Peptide Pen: Step-by-Step Guide


1. Supplies You’ll Need

  • Peptide pen (pre-filled or mixed)
  • Pen needles (single-use)
  • Alcohol wipes
  • Cotton ball or tissue
  • Sharps container (or a hard plastic bottle like a Gatorade bottle with a secure lid)

2. Getting Ready

  1. Wash your hands thoroughly with soap and water.
  2. Gather your supplies on a clean surface.
  3. Wipe the injection site (stomach or thigh) with an alcohol wipe and let it dry.

3. Attaching the Needle

  1. Remove the pen cap.
  2. Take a new needle, remove the protective seal, and screw or click it onto the pen tip.
  3. Pull off the outer cap and save it (you’ll need it later to remove the needle).
  4. Pull off the inner needle cover and discard it.

4. Priming the Pen (First Use Only)

  • Dial to 2 units and press the injection button with the pen pointing upward until a drop appears.
  • This ensures there is no air in the needle.

5. Setting Your Dose

  • Turn the dial on the pen to your prescribed dose, per your prescribers instructions

6. Giving the Injection

  1. Pinch a fold of skin on your lower belly (2 inches away from the belly button) or thigh.
  2. Insert the needle at a 90° angle into the pinched skin.
  3. Press the button all the way down until the dose counter returns to “0.”
  4. Hold the pen in place for 5–10 seconds to ensure the full dose is delivered.
  5. Remove the needle straight out.

7. After the Injection

  1. Press lightly on the site with a cotton ball or tissue (do not rub).
  2. Replace the outer cap on the needle and carefully unscrew it from the pen.
  3. Place the used needle into your sharps container (or hard plastic bottle with a screw-top lid, such as a Gatorade bottle).
  4. Store your pen in the refrigerator (do not freeze, do not store in the door).

8. Disposal of Needles

  • When your container is about ¾ full, tape the lid shut securely.
  • Label: “Used Sharps – Do Not Recycle”.
  • Dispose according to your local pharmacy or health department guidelines.

Pro Tips:

  • Rotate injection sites to prevent irritation.
  • Always use a new needle for each injection.
  • Take your injection at the same time daily (typically at bedtime).